Evaluation of estrogen receptor expression by <sup>18</sup>F-fluoroestradiol PET/CT imaging in human breast cancer model

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Evaluation of estrogen receptor expression by ¹⁸F-fluoroestradiol PET/CT imaging in human breast cancer model

更新时间：2025-12-15

- Evaluation of estrogen receptor expression by ¹⁸F-fluoroestradiol PET/CT imaging in human breast cancer model
- Vol. 24, Issue 1, Pages: 35-40(2015)
- 作者机构：
  
  1. 复旦大学生物医学影像研究中心,上海,200032
  2. 上海分子影像探针工程技术研究中心（筹）,上海,200032
  3. 复旦大学附属肿瘤医院核医学科，复旦大学上海医学院肿瘤学系,上海,200032
  4. 上海市质子重离子医院核医学科,上海,201321
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published Online：27 August 2015，
  
  Published：27 August 2015
- 稿件说明：
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何思敏,罗健民,张建平,等. ¹⁸F-FES小动物PET/CT评价不同乳腺癌模型雌激素受体表达的差异[J]. 肿瘤影像学, 2015, 24(1): 35-40
DOI：

何思敏,罗健民,张建平,等. ¹⁸F-FES小动物PET/CT评价不同乳腺癌模型雌激素受体表达的差异[J]. 肿瘤影像学, 2015, 24(1): 35-40 DOI：

摘要

目的：

用16-

18

F-17-雌二醇(

18

F-FES)小动物PET/CT评价不同类型乳腺癌模型雌激素受体(ER)表达的差异。

方法：

用ER阳性人乳腺癌细胞(ZR-75-1、MCF-7)和阴性细胞(MDA-MB-231)构建荷瘤裸鼠动物模型。阳性组每组各10只，接种前3 d植入雌激素缓释片，再将细胞与基质胶混合液接种于乳腺脂肪垫，成瘤后显像前3 d取出缓释片。阴性组5只，直接将细胞悬液接种于乳腺脂肪垫。当肿瘤长至长径5 mm左右时行

18

F-FES PET/CT显像，用%ID/g

max

定量ER表达，然后用免疫组化测定并比较ER结果。数据分析采用单因素方差分析。

结果：

ZR-75-1、MCF-7和MDA-MB-231的瘤体成瘤率分别为100％(10/10)、70%(7/10)和100％(5/5)，瘤体随时间延长而增大，但三组之间差异无统计学意义(

P

＞0.05)，

18

F-FES %ID/gmax分别为6.61.0、3.30.5和1.10.1，三组之间差异均有统计学意义(

P

＜0.05)。T/M比值分别为4.20.3、2.60.2和1.10.1，三组之间差异均有显著统计学意义(

P

＜0.001)。

18

F-FES显像结果与免疫组化结果呈正相关(%ID/g

max

：

r

2

=0.65，

P

＜0.001；T/M：

r

2

=0.87，

P

＜0.001)。

结论：

18

F-FES PET/CT的%ID/g

max

能准确评价不同类型荷人乳腺癌模型的ER表达水平，从而为进一步研究乳腺癌内分泌疗效提供了一种活体、无创、动态的分析方法。

Abstract

Objective:

To evaluate estrogen receptor (ER) expression level in human breast cancer model using

18

F-fluoroestradiol (

18

F-FES) microPET/CT.

Methods:

ER

+

human

breast cancer cell lines (ZR-75-1 and MCF-7) and ER- control (MDA-MB-231) were used to construct BALB/c nude mouse model. There were ten mice in each ER

+

group. The estrogen pellets were implanted 3 d prior to tumor cell inoculation and removed 3 d before administration of

18

F-FES. The mice were implanted with ZR-75-1 and MCF-7. There were 5 mice in ER- group. MDA-MB-231 cells were inoculated into thoracic mammary fat pad.

18

F-FES PET/CT was performed until the tumor length reached 5 mm. %ID/g

max

was used to quantitate ER expression. ER expression was also detected by immunohistochemistry.

Results:

The tumor formation rates for ZR-75-1

MCF-7 and MDAMB- 231 were 100% (10/10)

70% (7/10) and 100% (5/5)

respectively. There was no statistical difference in the volume among three groups (

P

0.05). %ID/g

max

values were 6.61.0

3.30.5 and 1.10.1

respectively. Significant differences were observed when ZR-75-1 was compared to MCF-7 (

P

0.05) and MDA-MB-231 (

P

0.001). There was significant difference between MCF- 7 and MDA-MB-231 (

P

0.05). The ratios of tumor to muscle (T/M) were 4.20.3

2.60.2 and 1.10.1

respectively. There were significant differences among three groups (

P

0.001). A significantly positive correlation was found between the resultsof 18F-FES imaging and immunohistochemistry (%ID/g

max

:

r

2

=0.65

P

0.001; T/M:

r

2

=0.87

P

0.001).

Conclusion:

%ID/g

max

from

18

F-FES PET/CT imaging is able to evaluate ER expression reliably.

1

8F-FES PET/CT can be used as a noninvasive and dynamic method in vivo.

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