Application of vascular endothelial growth factor receptor 2-targeted ultrasound molecular imaging in intraoperative assessment of tumor angiogenesis and boundary identification <em>in vivo</em>

ZHANG Haixian; LI Yiming; WANG Xiaohong; SHAO Jie; WANG Yi

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Application of vascular endothelial growth factor receptor 2-targeted ultrasound molecular imaging in intraoperative assessment of tumor angiogenesis and boundary identification in vivo

更新时间：2025-12-15

- Application of vascular endothelial growth factor receptor 2-targeted ultrasound molecular imaging in intraoperative assessment of tumor angiogenesis and boundary identification in vivo
- Vol. 25, Issue 3, Pages: 248-251(2016)
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- Published Online：02 November 2016，
  
  Published：02 November 2016
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张海娴,李一鸣,汪晓虹,等. 靶向血管内皮生长因子受体2超声分子成像在小鼠原位胶质瘤血管新生和边界识别中的应用[J]. 肿瘤影像学, 2016, 25(3): 248-251
DOI：

张海娴,李一鸣,汪晓虹,等. 靶向血管内皮生长因子受体2超声分子成像在小鼠原位胶质瘤血管新生和边界识别中的应用[J]. 肿瘤影像学, 2016, 25(3): 248-251 DOI：

摘要

目的：

探讨携带抗血管内皮生长因子受体2(vascular endothelial growth factor receptor 2，VEGFR2)单克隆抗体的超声微泡造影剂(microbubble，MB)在评价小鼠原位胶质瘤血管新生和边界识别中的作用。

方法：

以生物素-亲和素桥接法构建靶向微泡(MBv)，体外鉴定其性质。建立小鼠GL261胶质瘤模型，分别注射MBv和普通微泡(MBc)进行超声造影检查。

结果：

成功构建偶联抗小鼠VEGFR2单克隆抗体的MBv，造影结果表明MBv较MBc能更好地评价小鼠胶质瘤血管新生和肿瘤边界。

结论：

MBv是良好的肿瘤靶向造影剂，应用MBv可较好地实现术中评价小鼠胶质瘤血管新生，更好地辅助术中超声导航。

Abstract

Objective:

To develop and validate a targeted ultrasonographic (US) imaging agent with microbubbles (MBs) that binds to vascular endothelial growth factor receptor 2 (VEGFR2) in a murine model for tumor angiogenesis.

Methods:

Targeted US imaging agent was prepared by bridged avidin biotin through binding anti-VEGFR2 antibody to the shell of perfluorocarbonfilled MBs. The binding specificities of targeted MB (MBv) and with nonlabeled MB (MBc) were tested with VEGFR2-positive cells (human umbilical vein endothelial cells

HUVECs). In vivo imaging signals of contrast-enhanced US by using anti-VEGFR2-targeted MBv and MBc were quantified

in 10 mice bearing GL261 cells.

Results:

Attachment of HUVECs was significantly higher for MBv (mean MBs per cell 9.50.5) than MBc (mean MBs per cell 1.50.3). The imaging signal in the murine tumor angiogenesis model was significantly higher for MBv than for MBc.

Conclusion:

Targeted contrast-enhanced US directed at VEGFR2 improves in vivo visualization of tumor angiogenesis and boundary identification in a murine model of orthotopic gliomas.

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Related Institution

Department of Ultrasound, Shanghai Chest Hospital, Shanghai Jiao Tong University School of Medicine

Department of Ultrasound, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine

Department of Ultrasound, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University

Department of Ultrasound, Shanghai Jiao Tong University School of Medicine Affiliated Xinhua Hospital

Department of Pathology, Shanghai Jiao Tong University School of Medicine Affiliated Xinhua Hospital

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Application of vascular endothelial growth factor receptor 2-targeted ultrasound molecular imaging in intraoperative assessment of tumor angiogenesis and boundary identification in vivo

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摘要

Abstract

关键词

Keywords

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