乳腺癌淋巴微转移模型的建立及皮内与静脉注射<sup>18</sup>F-脱氧葡萄糖的诊断效果

李永霞; 郑营营; 孙玉云; 何思敏; 罗建民; 张建平; 曹天野; 王明伟; 章英剑

doi:10.19401/j.cnki.1007-3639.2018.03.007

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乳腺癌淋巴微转移模型的建立及皮内与静脉注射¹⁸F-脱氧葡萄糖的诊断效果

论著 | 更新时间：2025-12-15

- 乳腺癌淋巴微转移模型的建立及皮内与静脉注射¹⁸F-脱氧葡萄糖的诊断效果
- Establishing and diagnosis of lymph node micrometastasis with subcutaneous or intravenous injection of 18F-FDG by PET/CT imaging in mouse breast cancer
- 肿瘤影像学 2018年27卷第3期页码：164-168
- 作者机构：
  
  1. 上海市质子重离子医院核医学科,上海,201315
  2. 复旦大学附属肿瘤医院核医学科，复旦大学上海医学院肿瘤学系,上海,200032
  3. 复旦大学生物医学影像研究中心,上海,200032
  4. 上海分子影像探针工程技术研究中心,上海,200032
- 作者简介：
- 基金信息：
  
  国家自然科学基金青年科学基金（81401514）。
- DOI：10.19401/j.cnki.1007-3639.2018.03.007
  中图分类号：
- 网络出版：2018-07-26，
  
  纸质出版：2018-07-26
- 稿件说明：
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李永霞,郑营营,孙玉云,等. 乳腺癌淋巴微转移模型的建立及皮内与静脉注射¹⁸F-脱氧葡萄糖的诊断效果[J]. 肿瘤影像学, 2018, 27(3): 164-168 https://doi.

org/10.19401/j.cnki.1007-3639.2018.03.007
李永霞,郑营营,孙玉云,等. 乳腺癌淋巴微转移模型的建立及皮内与静脉注射¹⁸F-脱氧葡萄糖的诊断效果[J]. 肿瘤影像学, 2018, 27(3): 164-168 https://doi. DOI： 10.19401/j.cnki.1007-3639.2018.03.007.

org/10.19401/j.cnki.1007-3639.2018.03.007 DOI：

摘要

目的：

探讨乳腺癌小鼠区域性局部皮内和静脉注射

F-脱氧葡萄糖（

F-fluorodeoxyglucose，

F-FDG）后瘤周淋巴结PET/CT成像的差异，评估

F-FDG在肿瘤淋巴结微转移中的成像效果。

方法：

构建4T1原位乳腺癌淋巴结微转移模型，区域性局部皮内和次日静脉注射

F-FDG，进行小动物PET/CT显像，对淋巴结摄取

F-FDG较高者隔日再行瘤周局部皮内注射锝硫胶体（technetium sulfur colloid，

99m

Tc-SC）后行小动物SPECT/CT成像。显像完成后，取淋巴结组织进行H-E染色，以及上皮细胞角蛋白5/6（cytokeratin 5/6，CK5/6）和葡萄糖转运蛋白1（glucose transporter-1，Glut-1）免疫组织化学染色。

结果：

局部皮内注射时，有微转移的小鼠瘤周淋巴结摄取

F-FDG异常增高，ID%/g值为19.22.0（

=2），显著高于对照（自身对照时对侧淋巴结；ID%/g值为6.80.4）和无转移小鼠（ID%/g值为7.20.4）相同位置淋巴结（

0.001）。静脉注射时，未发现任何小鼠淋巴结摄取异常增高（ID%/g值为2.50.5；

=0.870）。局部皮内注射

99m

Tc-SC 时，可观测到多个腋淋巴结，其中1个瘤周淋巴结与

F-FDG高摄取淋巴结位置相吻合。H-E染色和CK5/6免疫组织化学染色证实，

F-FDG高摄取的淋巴结为发生微转移的淋巴结。

结论：

区域性局部皮内注射

F-FDG能定性前哨淋巴结，比静脉注射法早期发现肿瘤微转移淋巴结。

Abstract

Objective:

To investigate the difference in the identification of lymph node micrometastasis be

tween regional subcutaneous injection and intravascular injection of

F-fluorodeoxyglucose (

F-FDG) by PET/ CT imaging in mouse breast cancer.

Methods:

The mouse 4T1 breast cancer lymph node micrometastasis model was established. The regional subcutaneous and intravascular injection of 18F-FDG was performed for PET/CT imagingin two day

respectively. For the higher

F-FDG uptake in lymph node of mice

SPECT/CT imaging was also performed by subcutaneous injection of technetium sulfur colloi (

99m

Tc-SC). H-E staining and immunohistochemistry of epithelial cytokeratin 5/6 (CK5/6) and glucose transporter 1 (Glut-1) were measured.

Results:

The uptake value (ID%/g) of

F-FDG in lymph nodes was 19.22.0 (

=2) by subcutaneous injection in micrometastasis group

which was significantly higher than the contralateral ones (ID%/g of 6.80.4) and those without micrometastasis (ID%/g of 7.20.4) (

0.000 1). No increased uptake of

F-FDG was observed in any mouse lymph node (ID%/ g of 2.50.5) (

=0.870) by intravascular injection. Moreover

SPECT/CT imaging with 99mTc-SC revealed multiple axillary lymph nodes

one of which had a well-matched lymph node with high

F-FDG uptake. H-E staining and immunohistochemistry of CK5/6 confirmed that high

F-FDG uptake in lymph nodes revealed micrometastatic.

Conclusion:

Regional subcutaneous injection of

F-FDG can qualitatively identify micrometastatic sentinel lymph nodes earlier than intravenous injection

which is worthy of clinical application.

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Establishing and diagnosis of lymph node micrometastasis with subcutaneous or intravenous injection of 18F-FDG by PET/CT imaging in mouse breast cancer

DOI：10.19401/j.cnki.1007-3639.2018.03.007

摘要

Abstract

关键词

Keywords

references

乳腺癌淋巴微转移模型的建立及皮内与静脉注射¹⁸F-脱氧葡萄糖的诊断效果