Nb <sub>0.12</sub> -TiO <sub>2 </sub>对脑胶质瘤的光热治疗及乏氧微环境的影响

孙娜; 耿鹏; 赵凌舟; 龚佳丽; 邢岩; 陈志钢; 赵晋华

doi:10.19732/j.cnki.2096-6210.2021.03.002

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Nb _0.12 -TiO ₂对脑胶质瘤的光热治疗及乏氧微环境的影响

论著 | 更新时间：2025-12-15

- Nb _0.12 -TiO ₂对脑胶质瘤的光热治疗及乏氧微环境的影响
- Effects of Nb _0.12 -TiO ₂on the photothermal therapy and hypoxic microenvironment of glioma
- 肿瘤影像学 2021年30卷第3期页码：149-154
- 作者机构：
  
  1. 上海交通大学附属第一人民医院核医学科,上海
  2. 东华大学材料科学与工程学院,上海
- 作者简介：
- 基金信息：
  
  国家自然科学基金（81801727）
- DOI：10.19732/j.cnki.2096-6210.2021.03.002
  中图分类号：
- 网络出版：2021-06-28，
  
  纸质出版：2021-06-28
- 稿件说明：
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孙娜,耿鹏,赵凌舟,等. Nb _0.12 -TiO ₂对脑胶质瘤的光热治疗及乏氧微环境的影响[J]. 肿瘤影像学, 2021, 30(3): 149-154 https://doi.

org/10.19732/j.cnki.2096-6210.2021.03.002
孙娜,耿鹏,赵凌舟,等. Nb _0.12 -TiO ₂对脑胶质瘤的光热治疗及乏氧微环境的影响[J]. 肿瘤影像学, 2021, 30(3): 149-154 https://doi. DOI： 10.19732/j.cnki.2096-6210.2021.03.002.

org/10.19732/j.cnki.2096-6210.2021.03.002 DOI：

摘要

目的：

探讨新型光敏纳米材料铌-二氧化钛（Nb

0.12

-TiO

）在近红外光（near-infrared light，NIR）激发下对脑胶质瘤的光热治疗效果和对低氧诱导因子-1（hypoxia inducible factor-1，HIF-1）的影响。

方法：

使用细胞计数试剂盒-8（cell counting kit-8，CCK-8）法评估Nb

0.12

-TiO

在无NIR激发下对人脑胶质瘤细胞（U87细胞）活性的影响；活死细胞染色评估Nb

0.12

-TiO

对U87细胞的光热治疗效果；实时荧光定量聚合酶链反应（real-time fluorescence quantitative polymerase chain reaction，RTFQ-PCR）评估U87细胞经Nb

0.12

-TiO

光热治疗后HIF-1的基因表达水平。

结果：

CCK-8法结果表明，Nb

0.12

-TiO

与U87细胞共培养24 h后，细胞活性＞90%。活死细胞染色结果显示，在磷酸盐缓冲生理盐水（phosphate-buffered saline，PBS）组、Nb

0.12

-TiO

组和PBS+NIR组未出现明显的细胞凋亡，而Nb

0.12

-TiO

+NIR组出现大量细胞死亡。RTFQ-PCR实验发现，U87细胞经Nb

0.12

-TiO

+NIR治疗后，HIF-1的基因表达大幅下调，与PBS组、Nb

0.12

-TiO

组和PBS+NIR组差异有统计学意义（

＜0.001）。

结论：

0.12

-TiO

具有高度的生物相容性，在NIR激发下可显著杀伤U87细胞，且可同时下调HIF-1的表达，有望用于脑胶质瘤的光热治疗。

Abstract

Objective:

To evaluate the photothermal therapy effects of a new types of photosensitive nanomaterial

niobium-doped titanium dioxide (Nb

0.12

-TiO

)

on gliomas under the excitation of near-infrared light (NIR) and the effects of Nb

0.12

-TiO

on hypoxia inducible factor-1 (HIF-1).

Methods:

Cell counting kit-8 (CCK-8) assays was used to evaluate the effects of Nb

0.12

-TiO

on the viability of human glioma cells (U87 cells) without NIR stimulation. The photothermal effect of Nb

0.12

-TiO

on U87 cells was evaluated by live and dead cell double staining. The gene level of HIF-1 in U87 cells after photothermal treatment with Nb

0.12

-TiO

was evaluated by real-time fluorescence quantitative polymerase chain reaction (RTFQ-PCR).

Results:

CCK-8 assay showed that after cocultured with Nb

0.12

-TiO

for 24 h

the viability of U87 cells was ＞90%. The results of live and dead cell double staining showed that a large number of cell death occurred in Nb

0.12

-TiO

+NIR group

and there was no obvious apoptosis in phosphate-buffered saline (PBS) group

0.12

-TiO

group and PBS+NIR group. RTFQ-PCR showed that the expression of HIF-1 was significantly downregulated in U87 cells after treated with Nb

0.12

-TiO

+NIR

which was significantly different from that in PBS group

0.12

-TiO

group

and PBS+NIR group (

＜0.001).

Conclusion:

0.12

-TiO

with high biocompatibility

can significantly kill U87 cells and downregulate the expression of HIF-1 under the excitation of NIR

which is expected to be used in the photothermal treatment of glioma in future.

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Nb 0.12 -TiO 2 对脑胶质瘤的光热治疗及乏氧微环境的影响

Effects of Nb 0.12 -TiO 2 on the photothermal therapy and hypoxic microenvironment of glioma

DOI：10.19732/j.cnki.2096-6210.2021.03.002

摘要

Abstract

关键词

Keywords

references

Nb _0.12 -TiO ₂对脑胶质瘤的光热治疗及乏氧微环境的影响

Effects of Nb _0.12 -TiO ₂on the photothermal therapy and hypoxic microenvironment of glioma