动态三维超声造影评价瑞格非尼抑制兔肝VX <sub>2</sub>残癌增殖的实验研究

陈凯玲; 王坤; 毛丽娟; 毛枫; 韩红; 曹佳颖; 张小龙; 王文平

doi:10.19732/j.cnki.2096-6210.2024.02.009

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动态三维超声造影评价瑞格非尼抑制兔肝VX ₂残癌增殖的实验研究

论著 | 更新时间：2025-12-15

- 动态三维超声造影评价瑞格非尼抑制兔肝VX ₂残癌增殖的实验研究
- Experimental study of dynamic three-dimensional contrast-enhanced ultrasound in evaluating the inhibitory effect of regorafenib on the proliferation of incomplete ablation residual liver VX ₂ tumor
- 肿瘤影像学 2024年33卷第2期页码：156-162
- 作者机构：
  
  复旦大学附属中山医院超声科,上海,200032
- 作者简介：
- 基金信息：
  
  国家自然科学基金（82272013）;上海市自然科学基金（20ZR1452800）;上海申康中心重大临床研究项目（SHDC2020CR1031B）
- DOI：10.19732/j.cnki.2096-6210.2024.02.009
  中图分类号：
- 网络出版：2024-05-11，
  
  纸质出版：2024-05-11
- 稿件说明：
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陈凯玲,王坤,毛丽娟,等. 动态三维超声造影评价瑞格非尼抑制兔肝VX ₂残癌增殖的实验研究[J]. 肿瘤影像学, 2024, 33(2): 156-162 https://doi.

org/10.19732/j.cnki.2096-6210.2024.02.009
陈凯玲,王坤,毛丽娟,等. 动态三维超声造影评价瑞格非尼抑制兔肝VX ₂残癌增殖的实验研究[J]. 肿瘤影像学, 2024, 33(2): 156-162 https://doi. DOI： 10.19732/j.cnki.2096-6210.2024.02.009.

org/10.19732/j.cnki.2096-6210.2024.02.009 DOI：

摘要

目的：

观察瑞格非尼对不全消融残癌的抑制作用并探讨动态三维超声造影（three-dimensional contrast-enhanced ultrasound，3D-CEUS）评估靶向治疗反应的可行性。

方法：

构建兔VX

肝原位种植瘤模型及不全消融模型，随机将实验兔分为4组（

=5）：不全消融组（A组）、不全消融+靶向治疗组（B组）、靶向治疗组（C组）、对照组（D组）。在不全消融治疗前当天及治疗后7、14、21、28 d进行3D-CEUS检查并进行在机定量参数分析，实验结束后取出VX

肿瘤行细胞增殖指数（Ki-67）、CD34免疫组织化学染色，计算微血管密度（microvessel density，MVD）并比较各组间差异。

结果：

在治疗后21和28 d，B组和C组的峰值强度（peak intensity，PI）显著低于治疗前（

值分别为0.046，0.033）；而且，B组和C组的PI与D组相比差异有统计学意义（

值分别为0.027，0.031）。在治疗后28 d，B组和C组的上升支斜率（S）显著低于同时期对照组（

值分别为0.035

0.014）；而且B组与A组相比差异有统计学意义（

=0.027）。治疗后28 d，各组肿瘤组织Ki-67增殖指数、MVD计数差异无统计学意义（

＞0.05）

结论：

3D-CEUS定量分析技术可反映瑞格非尼抑制肝VX

原位种植瘤残癌增殖的治疗效果。

Abstract

Objective:

To observe the feasibility of three-dimensional contrast-enhanced ultrasound (3D-CEUS) in evaluating the inhibitory effect of regorafenib on incomplete ablation of residual c

ancer.

Methods:

The rabbits were randomly divided into 4 groups (

=5): incomplete ablation group (group A)

incomplete ablation + targeted therapy group (group B)

targeted therapy group (group C)

and control group (group D). 3D-CEUS was performed on the day before treatment and 7

28 d after treatment

and quantitative parameters were analyzed in vivo. VX

tumors were taken out at the end of the experiment for cell proliferation index (Ki-67) and CD34 immunohistochemical staining

and microvessel density (MVD) was calculated.

Results:

At 21 and 28 days after treatment

the peak intensity (PI) of group B and C was significantly lower than that before treatment (

=0.046

0.033

respectively). Moreover

the PI of group B and group C were significantly different from that of group D (

=0.027

0.031

respectively). At 28 d after treatment

the ascending slope (S) of group B and group C was significantly lower than that of the control group (

=0.035

0.014

respectively). There was significant difference between group B and group A (

=0.027). At 28 d after treatment

there was no significant difference in Ki-67 proliferation index and MVD among the three groups (

＞0.05).

Conclusion:

Quantitative analysis parameters of 3D-CEUS can reflect the efficacy of regorafenib in inhibiting the proliferation of residual cancer after incomplete ablation of VX

in situ orthotopic tumor.

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Experimental study of dynamic three-dimensional contrast-enhanced ultrasound in evaluating the inhibitory effect of regorafenib on the proliferation of incomplete ablation residual liver VX 2 tumor

DOI：10.19732/j.cnki.2096-6210.2024.02.009

摘要

Abstract

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Keywords

references

动态三维超声造影评价瑞格非尼抑制兔肝VX ₂残癌增殖的实验研究

Experimental study of dynamic three-dimensional contrast-enhanced ultrasound in evaluating the inhibitory effect of regorafenib on the proliferation of incomplete ablation residual liver VX ₂ tumor